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Correct operation process of biosafety cabinet

The secondary biosafety cabinet is the main clean equipment in the biological laboratory. It can prevent the harmfulness of toxic and harmful air suspended particles to the operator of the test, so as to ensure the safety of the operator and the environment in the process of the test.
 
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Correct operation of biosafety cabinet can provide certain protection for operators, products and the environment. It can avoid the damage caused by infectious substances and pollution in the experimental process. It is one of the most important clean equipment in laboratory equipment.
 
1. Close the front window and open the ultraviolet lamp for sterilization for 30 minutes before starting the machine. At this time, the operator and other personnel should avoid causing harm. Then the fan runs for 5 minutes and closes the exhaust (drainage) valve at the bottom of the cabinet to ensure the air flow stability of the safety cabinet.
 
2. Before working, first turn off the ultraviolet lamp, and then the operator should adjust the chair to a comfortable height to ensure that his face is above the front glass window and his arm enters the safe cabinet for about 1 minute before he can operate. This will enable the safe cabinet to resume the airflow state and allow the airflow to "wash out" the microorganisms on the surface of the arm. When the operator's arm is laid flat and operated on the front grille of the working area, some air flow will flow directly to the working area instead of entering the negative pressure channel. The problem can be solved by lifting the arm slightly. Therefore, the front grille should not be placed such as test records, waste plastic packaging, various equipment and other items, to ensure that the air flow through the grille into the negative pressure channel smoothly.
 
3. In the use of safety cabinet, the front glass plate should be in working state. It is suggested that the opening height should be slightly less than 20 cm.
 
4. The equipment and equipment used in the safety cabinet should be reduced to the minimum, and the air flow in the rear and both sides should not be blocked. There should be a corresponding gap between the equipment and other items.
 
5. Bunsen lamps are not allowed to be used in safety cabinets. A small electronic "furnace" can be used, and its power should be less than 1000W. It is recommended to use a sterile one-time inoculation ring.
 
6. Operators should not frequently enter and exit the working area of biosafety cabinet when they are working, otherwise they will disturb the airflow, destroy the protective air curtain and cause injury and pollution to personnel and environment.
 
7. When operating in safety cabinet, some measures should be taken to avoid cross-contamination. For example, open test tubes and bottles should not be placed vertically. Operators should cover them with a cover when using Petri dishes and tissue Petri dishes. (Vertical pipette collecting containers should not be used in biosafety cabinets.) In order to reduce the direct impact of downdraft on the open sterile surface, the cap and test tube cap should be re-capped as soon as possible after completion of operation.
 
8. After use, leave about 10 minutes for the self-cleaning time of the working area, then turn off the fan and turn on the ultraviolet lamp for sterilization for about 30 minutes, then complete the work and cut off the power supply to leave the laboratory.

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